Assistant Professor |School of Medicine   Research Group

Ruilin Tian is an assistant professor in School of Medicine, SUSTech. Ruilin completed his bachelor's degree in biological sciences at Peking University in 2015. He obtained his PhD degree from University of California, San Francisco (UCSF) in June 2020 in Dr. Martin Kampmann’s lab. After that, he continued his postdoc training in Martin’s lab. Ruilin joined SUSTech in the end of 2020.

During his PhD and postdoc, he pioneered a CRISPR-based functional genomics platform in human iPSC-derived neurons, which enables genome-wide modifier screens of disease-relevant cell biology to uncover disease mechanisms and identify novel therapeutic strategies.

The Tian lab’s research focuses on understanding cellular and molecular mechanisms of human diseases, especially neurodegenerative and neurodevelopmental disease, using innovative genomics and cell biology technologies. Our lab also develops new CRISPR-based functional genomics tools to facilitate our biomedical research.

Personal Profile

Research

1. Cellular and molecular mechanisms of human neurological diseases

2. Glia-neuron interaction

3. CRISPR-based functional genomics technology development

4. Single cell genomics/ systemic biology


Publications Read More

Hundley FV, Sanvisens Delgado N, Marin HC, Carr KL, Tian R, Toczyski DP. A comprehensive phenotypic CRISPR-Cas9 screen of the ubiquitin pathway uncovers roles of ubiquitin ligases in mitosis. Mol Cell. 2021 Jan 28:S1097-2765(21)00014-9.  PMID: 33539788.

Stephanie, S.,Chen, M., Bax, M., Tian, R.,…, Kampmann, M.(2020). PIKfyve inhibition blocks endolysosomal escape of α-synuclein fibrils and spread of α-synuclein aggregation. bioRxiv

Tian, R., Samelson, A., Rezelj, V., Chen, M., Ramadoss, G., Guo, X., … ,Liu, N. ,…,.Kampmann, M.(2020). BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2. bioRxiv

Tian, R.*, Abarientos, A., Hong, J., Hashemi, S. H., Yan, R., Nalls, M. A., … & Kampmann, M.* (2020). Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis. bioRxiv. (* corresponding authors)

Semesta, K. M., Tian, R., Kampmann, M., von Zastrow, M., & Tsvetanova, N. G. (2020). A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling. PLoS genetics16(10), e1009103.

Ramkumar, P., Abarientos, A. B., Tian, R., Seyler, M., Leong, J. T., Chen, M., … & Kampmann, M. (2020). CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma. Blood advances4(13), 2899-2911.

Guo, X., Aviles, G., Liu, Y., Tian, R., Unger, B.A., Lin, Y.H.T., Wiita, A.P., Xu, K., Correia, M.A. and Kampmann, M., (2020). Mitochondrial stress is relayed to the cytosol by an OMA1–DELE1–HRI pathway. Nature, 579(7799), pp.427-432.

Tian, R., Gachechiladze, M.A., Ludwig, C.H., Laurie, M.T., Hong, J.Y., …, Ward, M.E. & Kampmann, M., (2019). CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons. Neuron, 10.1016/j.neuron.2019.07.014

     – Featured as a cover story in Neuron,

     – Featured on the Neuron website with a video abstract

     – Featured by a Research Highlight in Nature Reviews Neuroscience.

 Ramkumar, P., Tian, R., Seyler, M., Leong, J. T., Chen, M., Choudhry, P., … & Kampmann, M., (2019). CRISPR-based screens uncover determinants of immunotherapy response and potential combination therapy strategies. bioRxiv, 833707.

Torres, S.E., Gallagher, C.M., Plate, L., Gupta, M., Liem, C.R., Guo, X., Tian, R., Stroud, R.M., Kampmann, M., Weissman, J.S. and Walter, P., (2019). Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether. eLife, 8.

Chen, J.J., Nathaniel, D.L., Raghavan, P., Nelson, M., Tian, R., …, and Kampmann, M., (2019). Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation. Journal of Biological Chemistry, pp.jbc-RA119.

Zhang, Y. J., Guo, L., … Tian,R., … & Petrucelli, L. (2019). Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly (PR) toxicity. Science363(6428), eaav2606.

Boettcher, M., Tian, R., Blau, J. A., Markegard, E., Wagner, R. T., Wu, D., … & McManus, M.T., (2018). Dual gene activation and knockout screen reveals directional dependencies in genetic networks. Nature biotechnology36(2), 170.

     – Featured by a News & Views in Nature Biotechnology

Mavor, D., Barlow, K. A., …, Tian, R., … , Kampmann, M. & Fraser, S. J., (2018). Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance. Biology open7(7), bio036103.

Pal, S., Lant, B., Yu, B., Tian, R., Tong, J., Krieger, J. R., … & Derry, W. B. (2017). CCM-3 promotes C. elegans germline development by regulating vesicle trafficking cytokinesis and polarity. Current Biology, 27(6), 868-876.

Xie, Z., Jay, K.A., Smith, D.L., Zhang, Y., Liu, Z., Zheng, J., Tian, R., Li, H. and Blackburn, E.H. (2015). Early telomerase inactivation accelerates aging independently of telomere length. Cell160(5), pp.928-939.

He, C., Tsuchiyama, S.K., Nguyen, Q.T., …, Tian, R., …, Kennedy, K.B. & Polymenis, M., (2014). Enhanced longevity by ibuprofen, conserved in multiple species, occurs in yeast through inhibition of tryptophan import. PLoS genetics10(12), p.e1004860.

Lab members Read More

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Contact Us

Contact Address

1088 Xueyuan Avenue, Shenzhen 518055, P.R. China

Office Phone

Email

tianrl@sustech.edu.cn

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