助理教授 |医学院   课题组网站

田瑞琳,南方科技大学医学院助理教授、博士生导师。2015年毕业于北京大学生物科学专业,2015年9月至2020年12月在美国加州大学旧金山分校(UCSF)取得博士学位,并进行博士后训练,2020年底加入南方科技大学医学院。

本课题组结合CRISPR基因表达调控系统(CRISPRi和CRISPRa)与人类诱导多能干细胞(iPSC)分化技术,开发出在人类神经元和神经胶质细胞中进行大规模遗传筛选的平台。本课题利用这一平台,并结合各种细胞分子生物学技术和单细胞测序等多组学方法,研究人类神经系统疾病的分子细胞生物学机制。

个人简介

研究领域

1. 神经发育疾病和神经退行性疾病的分子细胞生物学机制

2. 神经胶质细胞与神经元相互作用

3. 基于CRISPR和诱导多能干细胞(iPSC)的遗传筛选方法的开发

4. 单细胞组学/系统生物学


学术成果 查看更多

Hundley FV, Sanvisens Delgado N, Marin HC, Carr KL, Tian R, Toczyski DP. A comprehensive phenotypic CRISPR-Cas9 screen of the ubiquitin pathway uncovers roles of ubiquitin ligases in mitosis. Mol Cell. 2021 Jan 28:S1097-2765(21)00014-9.  PMID: 33539788.

Stephanie, S.,Chen, M., Bax, M., Tian, R.,…, Kampmann, M.(2020). PIKfyve inhibition blocks endolysosomal escape of α-synuclein fibrils and spread of α-synuclein aggregation. bioRxiv

 Tian, R., Samelson, A., Rezelj, V., Chen, M., Ramadoss, G., Guo, X., … ,Liu, N. ,…,.Kampmann, M.(2020). BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2. bioRxiv

Tian, R.*, Abarientos, A., Hong, J., Hashemi, S. H., Yan, R., Nalls, M. A., … & Kampmann, M.* (2020). Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis. bioRxiv. (* corresponding authors)

Semesta, K. M., Tian, R., Kampmann, M., von Zastrow, M., & Tsvetanova, N. G. (2020). A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling. PLoS genetics16(10), e1009103.

Ramkumar, P., Abarientos, A. B., Tian, R., Seyler, M., Leong, J. T., Chen, M., … & Kampmann, M. (2020). CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma. Blood advances4(13), 2899-2911.

Guo, X., Aviles, G., Liu, Y., Tian, R., Unger, B.A., Lin, Y.H.T., Wiita, A.P., Xu, K., Correia, M.A. and Kampmann, M., (2020). Mitochondrial stress is relayed to the cytosol by an OMA1–DELE1–HRI pathway. Nature, 579(7799), pp.427-432.

Tian, R., Gachechiladze, M.A., Ludwig, C.H., Laurie, M.T., Hong, J.Y., …, Ward, M.E. & Kampmann, M., (2019). CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons. Neuron, 10.1016/j.neuron.2019.07.014

     – Featured as a cover story in Neuron,

     – Featured on the Neuron website with a video abstract

     – Featured by a Research Highlight in Nature Reviews Neuroscience.

 

Ramkumar, P., Tian, R., Seyler, M., Leong, J. T., Chen, M., Choudhry, P., … & Kampmann, M., (2019). CRISPR-based screens uncover determinants of immunotherapy response and potential combination therapy strategies. bioRxiv, 833707.

Torres, S.E., Gallagher, C.M., Plate, L., Gupta, M., Liem, C.R., Guo, X., Tian, R., Stroud, R.M., Kampmann, M., Weissman, J.S. and Walter, P., (2019). Ceapins block the unfolded protein response sensor ATF6α by inducing a neomorphic inter-organelle tether. eLife, 8.

Chen, J.J., Nathaniel, D.L., Raghavan, P., Nelson, M., Tian, R., …, and Kampmann, M., (2019). Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation. Journal of Biological Chemistry, pp.jbc-RA119.

Zhang, Y. J., Guo, L., … Tian,R., … & Petrucelli, L. (2019). Heterochromatin anomalies and double-stranded RNA accumulation underlie C9orf72 poly (PR) toxicity. Science363(6428), eaav2606.

Boettcher, M., Tian, R., Blau, J. A., Markegard, E., Wagner, R. T., Wu, D., … & McManus, M.T., (2018). Dual gene activation and knockout screen reveals directional dependencies in genetic networks. Nature biotechnology36(2), 170.

     – Featured by a News & Views in Nature Biotechnology

Mavor, D., Barlow, K. A., …, Tian, R., … , Kampmann, M. & Fraser, S. J., (2018). Extending chemical perturbations of the ubiquitin fitness landscape in a classroom setting reveals new constraints on sequence tolerance. Biology open7(7), bio036103.

Pal, S., Lant, B., Yu, B., Tian, R., Tong, J., Krieger, J. R., … & Derry, W. B. (2017). CCM-3 promotes C. elegans germline development by regulating vesicle trafficking cytokinesis and polarity. Current Biology, 27(6), 868-876.

Xie, Z., Jay, K.A., Smith, D.L., Zhang, Y., Liu, Z., Zheng, J., Tian, R., Li, H. and Blackburn, E.H. (2015). Early telomerase inactivation accelerates aging independently of telomere length. Cell160(5), pp.928-939.

He, C., Tsuchiyama, S.K., Nguyen, Q.T., …, Tian, R., …, Kennedy, K.B. & Polymenis, M., (2014). Enhanced longevity by ibuprofen, conserved in multiple species, occurs in yeast through inhibition of tryptophan import. PLoS genetics10(12), p.e1004860.

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广东省深圳市南山区学苑大道1088号南方科技大学

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tianrl@sustech.edu.cn

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