1. 细菌VI型分泌系统作用机制与机理。

2. 细菌VI型分泌系统新型抗菌蛋白。

3. 病原毒力蛋白对宿主蛋白的修饰和调控。

4. 新冠病毒蛋白互作与生理功能。

主讲课程一：医学微生物学（本科）

主讲课程二：病原生物学前沿进展（研究生）

专注于肠道微生物研究，近五年来在Cell Host & Microbe、Molecular Cell、 Science Translational Medicine、P. Natl. Acad. Sci. USA、Journal of Immunology和Vaccine等国际权威学术期刊发表论文15篇，第一作者及通讯作者6篇。其中包括两篇Cell Host & Microbe及一篇PNAS。其工作被国内外多个媒体报道，受邀发表多次重要学术会议报告，获Fogarty Global Health Fellowship奖，担任Bio-Protocol杂志编委，Molecular Medicine Reports、mBio多家知名科学期刊审稿人。

已发表论文：

1、Liu M#, Zhao MY#, Wang H, Wang ZH, Wang Z, Liu Y, Li YP, Dong T, Fu Y. Pesticin-Like Effector VgrG3^cp Targeting Peptidoglycan Delivered by the Type VI Secretion System Contributes to Vibrio cholerae Interbacterial Competition. Microbiol Spectr. 2023 Feb 14;11(1):e0426722.(#为共同第一作者)

2、Feng Q, Zhou J, Zhang L, Fu Y, Yang L. Insights into the molecular basis of c-di-GMP signalling in Pseudomonas aeruginosa. Crit Rev Microbiol. 2022 Dec 20:1-19.

3、Zhang K#, Peng T#, Tao X#, Tian M#, Li Y, Wang Z, Ma S, Hu S, Pan X, Xue J, Luo J, Wu Q, Fu Y*, Li S*. Structural insights into caspase ADPR deacylization catalyzed by a bacterial effector and host calmodulin. Mol Cell. 2022 Nov 18:S1097-2765(22)01060-7. (#为共同第一作者，*为共同通讯作者)

4、Tang MX, Pei TT, Xiang Q, Wang ZH, Luo H, Wang XY, Fu Y, Dong T. Abiotic factors modulate interspecies competition mediated by the type VI secretion system effectors in Vibrio cholerae. ISME J. 2022 Jul;16(7):1765-1775.

5、Xiang, Q., Li, L., Wu, J., Tian, M., and Fu, Y. (2022). Application of pseudovirus system in the development of vaccine, antiviral-drugs, and neutralizing antibodies. Microbiological Research, 126993.

6、Zhang Y, Gao H, Hu X, Wang Q, Zhong F, Zhou X, Lin C, Yang Y, Wei J, Du W, Huang H, Zhou H, He W, Zhang H, Zhang Y, McCormick PJ, Fu J, Wang D, Fu Y, Lu X, Zhang T, Duan J, Qin B, Jiang H, Luo J, Zhang Y, Chen Q, Luo Q, Cheng L, Zhang Z, Zhang J, Li J. Structure-Based Discovery and Structural Basis of a Novel Broad-Spectrum Natural Product against the Main Protease of Coronavirus. J Virol. 2022 Jan 12;96(1):e0125321.

7、Song X, Li J, Tian M, Zhu H, Hu X, Zhang Y, Cao Y, Ye H, McCormick PJ, Zeng B, Fu Y, Duan J, Zhang J. Cryo-EM structure of mouse TRPML2 in lipid nanodiscs. J Biol Chem. 2022 Feb;298(2):101487.

8、Yu, K.-W., Xue, P., Fu, Y., and Yang, L. (2021). T6SS mediated stress responses for bacterial environmental survival and host adaptation. International Journal of Molecular Sciences 22, 478.

9、Li J, Zhou X, Zhang Y, Zhong F, Lin C, McCormick PJ, Jiang F, Luo J, Zhou H, Wang Q, Fu Y, Duan J, Zhang J. Crystal structure of SARS-CoV-2 main protease in complex with the natural product inhibitor shikonin illuminates a unique binding mode. Sci Bull (Beijing). 2021 Apr 15;66(7):661-663.

10、Zhou, X., Zhong, F., Lin, C., Hu, X., Zhang, Y., Xiong, B., Yin, X., Fu, J., He, W., and Duan, J. (2021). Structure of SARS-CoV-2 main protease in the apo state. Science China Life Sciences 64, 656-659.

11、Xue, J., Pan, X., Peng, T., Duan, M., Du, L., Zhuang, X., Cai, X., Yi, X., Fu, Y., and Li, S. (2020). Auto arginine-GlcNAcylation is crucial for bacterial pathogens in regulating host cell death. Frontiers in cellular and infection microbiology 10, 197.

12、Cai Q, Huang D, Ou P, Yu H, Zhu Z, Xia Z, Su Y, Ma Z, Zhang Y, Li Z, He Q, Liu L, Fu Y, Chen J. COVID-19 in a designated infectious diseases hospital outside Hubei Province, China. Allergy. 2020 Jul;75(7):1742-1752.

13、An J, Liao X, Xiao T, Qian S, Yuan J, Ye H, Qi F, Shen C, Wang L, Liu Y, Cheng X, Li N, Cai Q, Wang F, Chen J, Li G, Cai Q, Liu Y, Wang Y, Zhang F, Fu Y, He Q, Tan X, Liu L, Zhang Z. Clinical characteristics of recovered COVID-19 patients with re-detectable positive RNA test. Ann Transl Med. 2020 Sep;8(17):1084.

14、Duan, X., Fu, Y., and Yang, L. (2020). Molecular and systems biology approaches for analyzing drug-tolerant bacterial persister cells. In Sustainable Agriculture Reviews 46 (Springer, Cham), pp. 109-128.

15、Zhao J, Yuan Q, Wang H, Liu W, Liao X, Su Y, Wang X, Yuan J, Li T, Li J, Qian S, Hong C, Wang F, Liu Y, Wang Z, He Q, Li Z, He B, Zhang T, Fu Y, Ge S, Liu L, Zhang J, Xia N, Zhang Z. Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019. Clin Infect Dis. 2020 Nov 19;71(16):2027-2034.

16、Fu, Y., Ho, B.T., and Mekalanos, J.J. (2018). Tracking Vibrio cholerae cell-cell interactions during infection reveals bacterial population dynamics within intestinal microenvironments. Cell host & microbe 23, 274-281. e272.

17、Sanchez-Larrayoz, A.F., Elhosseiny, N.M., Chevrette, M.G., Fu, Y., Giunta, P., Spallanzani, R.G., Ravi, K., Pier, G.B., Lory, S., and Maira-Litrán, T. (2017). Complexity of complement resistance factors expressed by Acinetobacter baumannii needed for survival in human serum. The Journal of Immunology 199, 2803-2814.

18、Ho, B.T., Fu, Y., Dong, T.G., and Mekalanos, J.J. (2017). Vibrio cholerae type 6 secretion system effector trafficking in target bacterial cells. Proceedings of the National Academy of Sciences 114, 9427-9432.

19、Roux, D., Danilchanka, O., Guillard, T., Cattoir, V., Aschard, H., Fu, Y., Angoulvant, F., Messika, J., Ricard, J.-D., and Mekalanos, J.J. (2015). Fitness cost of antibiotic susceptibility during bacterial infection. Science translational medicine 7, 297ra114-297ra114.

20、Fu, Y., and Mekalanos, J.J. (2014). Infant Rabbit Colonization Competition Assays.

21、 Altindis, E., Fu, Y., and Mekalanos, J.J. (2014). Proteomic analysis of Vibrio cholerae outer membrane vesicles. Proceedings of the National Academy of Sciences 111, E1548-E1556.

22、Fu, Y., Waldor, M.K., and Mekalanos, J.J. (2013). Tn-Seq analysis of Vibrio cholerae intestinal colonization reveals a role for T6SS-mediated antibacterial activity in the host. Cell host & microbe 14, 652-663.

23、Liao, Q., Strong, A.J., Liu, Y., Liu, Y., Meng, P., Fu, Y., Touzjian, N., Shao, Y., Zhao, Z., and Lu, Y. (2012). HIV vaccine candidates generate in vitro T cell response to putative epitopes in Chinese-origin rhesus macaques. Vaccine 30, 1601-1608.