副教授 生物系
邓怿,副教授,博士生导师。1996年本科毕业于山东大学生物系,2002年于德国波鸿鲁尔大学(Ruhr-University Bochum)取得生物学博士学位,2003至2008年在美国国家卫生研究院(NICHD & NIDDK, NIH)从事博士后研究,主要进行细胞内囊泡运输方面的研究工作(合作导师 Dr . Cathy L. Jackson和Dr. Caroline C. Philpott)。2009至2011年在香港科技大学开展了细胞周期调控与衰老方面的研究 (合作导师 Prof. Randy YC Poon),2011-2012年在香港中文大学进行了短暂的糖尿病治疗方面的研究(合作导师 Prof. Gang Xu)。2012年受聘南方科技大学,建立了以研究细胞-基质黏附和细胞运动为中心的研究实验室。目前的研究主要通过细胞生物学(例如基于荧光技术的活细胞成像)和生物化学方法揭示细胞黏附、伸展和迁移的调控机制, 增进对肿瘤、纤维化疾病等疾病发生发展的认识。
个人简介
研究领域
本实验室主要的研究兴趣是细胞-基质黏附调控细胞运动、细胞生长的机制,目前正在开展的课题包括:
细胞-基质黏附调控细胞运动的机制。 整合素(integrin)介导的细胞-基质黏附是细胞感受细胞微环境(基质)的重要信号通路。异常的细胞-基质黏附与肿瘤转移等恶性疾病密切相关。基于前人在模式生物和其他真核细胞的研究,已发现许多多整合素相关的关键蛋白或蛋白复合物,如FAK、talin、kindlin、ILK-PINCH-parvin、RSU1等。
Kindlin是调控整合素活化的重要蛋白家族,包括三个成员,kindlin-1、-2、-3。Kindlin介导整合素“由内向外”(”inside-out“)信号起始,此外,还介导整合素“由外向内”(outside-in)信号传递。最近的研究中我们发现kindlin缺失抑制细胞-基质黏附(黏焯斑)解聚,影响细胞运动的协调,从而使得细胞运动的方向性变差。我们发现kindlin-2与MLCK相互作用,调控细胞的收缩力(actomyosin contractility)。通过单分子生物传感器,我们测量黏着斑的受力情况,提示了kindlin-2在肌球蛋白收缩力的局部调控中发挥作用。我们将深入研究kindlin对细胞群体运动(collective migration)的影响和调控机制,解析细胞-基质黏附如何调控细胞运动。这些研究涉及活细胞动态成像、生物传感器成像分析等。
细胞-基质黏附与肿瘤的发生和发展。在细胞-基质黏附的功能研究方面,细胞-基质黏附与肿瘤发生和发展的关系一直是我们的兴趣所在。众多的研究表明,正常细胞的生长和增值依赖于整合素介导细胞-基质黏附,而肿瘤细胞的生长则不依赖于细胞-基质黏附。我们发现,进化上十分保守的黏着斑蛋白RSU1负调控MEK-ERK信号通路,我们正在从肿瘤发生的基本原理出发,解析RSU1如何抑制MEK-ERK信号传导的分子机制。这些研究涉及生化和细胞生物学分析信号通路变化等。
整合素信号与血管生成。整合素信号传导调控肌动蛋白细胞骨架动态,从而影响相关的组织器官的发育和功能。我们发现,RSU1缺失的小鼠胚胎出现出血、水肿、发育迟缓和晚期胚胎致死表型,提示可能与血管生成或血管发育缺陷有关。我们正在开展血管发生方面的工作,旨在深入研究RSU1相关的功能和分子机制。这些研究涉及动物模型的建立和组织样本的分析。
教学
主讲本科生课程:
分子生物学 (BIO320)
细胞生物学(BIO206)
高级细胞生物学(BIO323)
生命科学概论(BIO102B)
参讲课程
生命科学前沿讲座(BIO308)
学术成果 查看更多
发表论文
publications at SUSTech
1. Wang CD, Guo XF, Wong TCB, Wang H, QI XF, Cai DQ, Deng Y, Zhao H (2019) Developmental expression of three prmt genes in Xenopus. Zool Res. Mar 18;40(2):102-107.
2. Shi Z, Xin H, Tian D, Lian J, Wang J, Liu G, Ran R, Shi S, Zhang Z, Shi Y, Deng Y, Hou C, Chen Y (2019) Modeling human point mutation diseases in Xenopus tropicalis with a modified CRISPR/Cas9 system. FASEB J. Mar 7: fj201802661R. doi: 10.1096/fj.201802661R. [Epub ahead of print]
3. Sun Y, Ding Y, Guo C, Liu C, Ma P, Ma S, Wang Z, Liu J, Qian T, Ma L, Deng Y, Wu C (2019) -Parvin promotes breast cancer progression and metastasis through interaction with G3BP2 and regulation of TWIST1 signaling. Oncogene. Feb 25. doi: 10.1038/s41388-019-0762-1. [Epub ahead of print].
4. Ke M, Liu J, Chen W, Chen L, Gao W, Qin Y, He A, Chu B, Tang J, Xu R, Deng Y#, Tian R# (2018) Integrated and Quantitative Proteomic Approach for Charting Temporal and Endogenous Protein Complexes. Anal Chem. 90: 12574. (# Corresponding author).
5. Li T*, Deng Y*, Shi Y, Tian R, Chen Y, Zou L, Kazi JU, Rönnstrand L, Feng B, Chan SO, Chan WY, Sun J, Zhao H (2018) Bruton’s tyrosine kinase potentiates ALK signaling and serves as a potential therapeutic target of neuroblastoma. Oncogene. Jul 16. doi: 10.1038/s41388-018-0397-7. [Epub ahead of print] (*co-first author).
6. Liu W, Zhong X, Cheng L, Wang J, Sun Y, Deng Y#, Zhang ZT# (2018) Cellular and compositional insight into the sludge dewatering process using enzyme treatment. Environ Sci Pollut Res Int. Aug 14. doi: 10.1007/s11356-018-2854-9. [Epub ahead of print] (#Corresponding authors).
7. Guo L, Cai T, Chen K, Wang R, Wang J, Cui C, Yuan J, Zhang K, Liu Z, Deng Y, Xiao G, Wu C (2018) Kindlin-2 regulates mesenchymal stem cell differentiation through control of YAP1/TAZ. J Cell Biol. 217: 1431.
8. Li H*, Deng Y*, Sun K, Yang H, Liu J, Wang M, Zhang Z, Lin J, Wu C, Wei Z, Yu C (2017) Structural basis of kindlin-mediated integrin recognition and activation. Proc Natl Acad Sci USA. 114: 9349. (*Co-first authors).
9. Liu Z, Kwan T, Cheng, Shi Z, Liu Z, Lei Y, Wang C, Shi W, Chen X, Qi X, Cai D, Feng B, Deng Y, Chen C and Zhao H (2016) Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos. Cell Biosci. 6: 22.
10. Wang C, Kam RK, Shi W, Xia Y, Chen X, Cao Y, Sun J, Du Y, Lu G, Chen Z, Chan WY, Chan SO, Deng Y, Zhao H (2015) The Proto-oncogene Transcription Factor Ets1 Regulates Neural Crest Development through Histone Deacetylase 1 to Mediate Output of Bone Morphogenetic Protein Signaling. J Biol Chem. 290: 21925.
11. Shi W, Xu G, Wang C, Sperber SM, Chen Y, Zhou Q, Deng Y#, Zhao H# (2015) Heat Shock 70-kDa Protein 5 (Hspa5) Is Essential for Pronephros Formation by Mediating Retinoic Acid Signaling. J Biol Chem. 290:577. (#Co-corresponding authors).
12. Lei Y, Guo X, Deng Y, Chen Y, Zhao H (2013) Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos. Cell Biosci. 3: 21.
13. Kang ZF, Deng Y, Zhou Y, Fan RR, Chan JC, Laybutt DR, Luzuriaga J, Xu G (2013) Pharmacological reduction of NEFA restores the efficacy of incretin-based therapies through GLP-1 receptor signalling in the beta cell in mouse models of diabetes. Diabetologia 56: 423.
14. Lei Y, Guo X, Liu Y, Cao Y, Deng Y, Chen X, Cheng CH, Dawid IB, Chen Y, Zhao H (2012) Efficient targeted gene disruption in Xenopus embryos using engineered transcription activator-like effector nucleases (TALENs). Proc Natl Acad Sci USA. 109: 17484.
publications prior to SUSTech
15. Kam RK, Deng, Y, Chen Y, Zhao H (2012) Retinoic acid synthesis and functions in early embryonic development. Cell Biosci. 2: 11.
16. Deng Y, Guo Y, Watson H, Au WC, Shakoury-Elizeh M, Basrai MA, Bonifacino JS, Philpott CC (2009) GGA2 mediates sequential ubiquitin-independent and -dependent steps in the trafficking of ARN1 from the trans-golgi network to the vacuole. J Biol Chem. 284: 23830.
17. Deng Y, Golinelli-Cohen M, Smirnova E, and Jackson CL (2009) A COPI coat subunit interacts directly with an early-Golgi localized Arf Exchange Factor. EMBO Rep. 10: 58.
18. Kim Y*, Deng Y*, Philpott CC (2007) GGA2- and Ubiquitin-dependent Trafficking of Arn1, the Ferrichrome Transporter of Saccharomyces cerevisiae. Mol Biol Cell. 18: 1790. (* co-first author).
19. Deng Y, Schmidtmann A, Kruse S, Filatov V, Heilmeyer LM Jr, Jaquet K Thieleczek (2003) Phosphorylation of human cardiac troponin I G203S and K206Q linked to familial hypertrophic cardiomyopathy affects actomyosin interaction in different ways. J Mol Cell Cardiol. 35: 1365.
20. Wang JX, Zhao XF, Deng Y, Qi HY, Wang ZJ (2003) Chromosomal polymorphism of mandarin vole, Microtus mandarinus (Rodentia). Hereditas 138: 47.
21. Wang JX, Zhao XF, Koh HS, Deng Y, Qi HY (2003) Chromosomal polymorphisms due to heterochromatin growth and pericentric inversions in white-bellied rat, Niviventer confucianus, from China. Hereditas 138: 59.
22. Deng Y, Schmidtmann A., Redlich A., Westerdorf B., Jaquet K, Thieleczek R (2001) Effects of Phosphorylation and Mutation R145G on Human Cardiac Troponin I Function. Biochemistry 40: 14593.